PCR product cloning

Joe Boutell boutell at cf.ac.uk
Fri Apr 25 07:24:04 EST 1997


We have used the Sureclone ligation kit from Pharmacia for this. One 
reaction to blunt and phosphorylate your PCR product, then blunt end 
ligation into SmaI site of pUC18, giving blue/white selection. Worked 
well enough for us to use our own homemade chemically competent cells.
Cheers,
Joe Boutell.




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