Mail at scri.sari.ac.uk Mail at scri.sari.ac.uk
Fri Apr 25 10:03:12 EST 1997

Dear all,

Has anyone out there got any experience using zeocin? We are trying to 
subclone a gene fragment into Invitrogens pGAPZ vector for expression in 
Pichia. I know that our ligation into the vector is working fine (checked it 
by PCR using sequencing primers) but after transformation most of our colonies 
appear to have no plasmid in. 
We're using low salt media, fresh antibiotic etc etc. 

Any suggestions gratefully recieved.

John Jones
(jjones at scri.sari.ac.uk)

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