Marisol Rodriguez Pena
pena at chem.vu.nl
Thu Apr 24 18:52:07 EST 1997
I have problems to detect an HA tagged receptor in a Western Blot. The
receptor is present in binding assays and the HA tag works fine in
I am expressing the receptor in COS cells, isolate membranes and I
load about 50 micrograms in a 10 % SDS-PAGE. The transfer to
nitrocelulose works fine but after ECL detection I am unable to detect
any specific band in the autorradiography.
Does anyone have some clue of what I am doing wrong?
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