Taqman probes help needed

hoolwerf at solair1.inter.nl.net hoolwerf at solair1.inter.NL.net
Mon Apr 28 09:26:34 EST 1997


"merel" <Patrick.Merel at Inforoute.cgs.fr> wrote:

 m> I am planning TaqMan developments in the medical diagnostic
 m> field. I should like to understand the way the TaqMan probes must
 m> be designed (length, Tm, fluorochromes, termination...ect). Is
 m> there any publication on this topic in the litterature?

Perkin Elmer should be able to give you all these information. At least they
gave us since we are planning a detection system.

PE is also able to help you to determine the primers and probe you need and as
far as i know you need PE for the double labelled probe.

with regards, Jan Hoolwerf

******************* these words were mine, all mine !! ********************
 J.D. Hoolwerf
 Neth. Inst. for Dairy Research
 P.O. Box 20                        phone  : 31-318-659511
 6710 BA  Ede                       fax    : 31-318-650400
 the Netherlands                    e-mail : hoolwerf at solair1.inter.nl.net

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