Hybridization with DD RT-PCR-product

Bodil Svanberg bodil.svanberg at FYSIOLOGI.GU.SE
Mon Apr 28 09:12:23 EST 1997


Using differential display of mRNA (DD RT-PCR) I have isolated several
cDNAs that I which to study further using northern hybridization. To my
surprise, some of the cDNAs hybridized to short fragments of 0-1000 bp,
when compared to the ribosomal bands seen on the gel. Has anyone
received the same results using DD RT-PCR? Could anyone tell me what the
hybridization product could be?

Grateful for any answer,

Bodil



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