33P and gel shifts

[J.Eriksson]

Len Levin wrote:
> 
> Has anyone (successfully) used 33P for their gel shifts? Any tips or warnings?
> What kind of exposure times? Gel thickness?
> 
> Thanks in advance for any help!
> 
> Leonard A. Levin, M.D., Ph.D.
> University of Wisconsin Medical School

I did gelshifts with P33 a couple of times. I dried the gel and exposed 
the gel for 24-36 hours in -80 C. It works better with P32, but its a bit 
nasty yo work with.