help protein concentration

Mark Bryant jmb36 at mole.bio.cam.ac.uk
Tue Apr 29 06:17:43 EST 1997


How can I concentrate a purified, recombinant His-tagged protein to near
10mg/ml without it precipitating out of solution? Any tips on buffers or
salts to try?
	Mark Bryant
	Dept. of Clinical Biochemistry
	University of Cambridge 




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