pfu for cloning PCR
zxz4 at PSUVM.PSU.EDU
Wed Apr 30 14:37:23 EST 1997
> Subject: pfu for cloning PCR?
> Date: 30 Apr 1997 17:08:55 GMT
> Hi all, does anybody know if pfu can be used for cloning PCR. I know
> is suitful for it. The reason I am using pfu is that I has tons of
> pfu. It
> has tried several times by many person in our lab in different
> experiments and seemed no success. I dont know if someone has similar
> problems as I have.
> Thanks everybody, I like you.
> C.Lu at cellbio.duke.edu
Dr. Duncan Clark has just summarized the polymerases used in PCR (see
"Re: Overhang A of PCR". 4/29). The PCR products generated by pfu are
supposed to be ligated into the vector with blunt ends since the enzyme
has proofreading activity. I do not quite get what you meant "no
success". Is it "no success" in PCR or ligation? If your PCR works with
pfu, the problems are in ligation.
Zhiyong Zhao, Ph.D.
More information about the Methods