bernard at elsie.nci.nih.gov
Wed Apr 30 02:49:43 EST 1997
In article <5k2mac$mqo at lyra.csx.cam.ac.uk>, rw200 at cus.cam.ac.uk says...
>Can anyone tell me any Dam methylase minus strains of E.coli.
>I need to be able to cut a Bcl1 site.
>Thanks very much
>Email rw200 at cus.cam.ac.uk
GM2163 Free from NEB, reasonably hardy
GM2929 Free from NEB, rec- (recF), less hardy
JM110 From various companies
SCS110 From Stratagene, endA- version of JM110
DM1 Gibco BRL
There are probably many others.
All are dam- dcm-. I've used GM2163 and it worked just fine.
I have no affiliation with any of the above companies.
Bernard Murray, Ph.D.
bernard at elsie.nci.nih.gov (National Cancer Institute, NIH, Bethesda MD, USA)
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