Dam-

[Bernard Murray]

In article <5k2mac$mqo at lyra.csx.cam.ac.uk>, rw200 at cus.cam.ac.uk says...
>
>Dear All,
>Can anyone tell me any Dam methylase minus strains of E.coli.
>I need to be able to cut a Bcl1 site.
>Thanks very much
>Robert
>Email rw200 at cus.cam.ac.uk

GM2163	Free from NEB, reasonably hardy
GM2929	Free from NEB, rec- (recF), less hardy
JM110	From various companies
SCS110	From Stratagene, endA- version of JM110
DM1	Gibco BRL
There are probably many others.

All are dam- dcm-.  I've used GM2163 and it worked just fine.
I have no affiliation with any of the above companies.

	Bernard

Bernard Murray, Ph.D.
bernard at elsie.nci.nih.gov  (National Cancer Institute, NIH, Bethesda MD, USA)