nuclear staining ISH

[harris]

ISH: we can demonstrate cytokine cytoplasmic presence in malignant cells by 
immunohistochem and Western blot. We can demonstrate mRNA for the same 
cytokine by RT-PCR. To date Northern blots have not been succesful. ISH 
analysis has revealed nuclear localisation of mRNA signal, with no 
cytoplasmic, repeatedly. We are confident there is no technical error in our 
method. We have repeated experiments following incubation with cycloheximide 
again with no success. Why should this be so? Are we dealing with simple low 
concentration presence of cytokine mRNA and a short half life or is the 
explanation more complex or incomprehensible?