His-tag purification

[F. Nardone]

Lynn Hughes wrote:
 Has
> anyone had experience with the various resins available and which are
> best?  I have come across ProBond Metal Binding Resin, Talon Metal
> Affinity Resin, His-Bind Resin and the Ni-NTA resin from Quiagen.  Any
> comments would be appreciated.
> 

In my lab we use Ni-NTA from Quiagen, it works fine in denaturing
conditions but I never got a clean purification in non-denaturing
conditions.