NP-40 replacement?

[John A. Newitt]

In article <3.0.32.19970725101517.00727500 at imm2.imm.uth.tmc.edu>,
dhavilan at IMM2.IMM.UTH.TMC.EDU ("David L. Haviland, Ph.D.") wrote:

> I've been doing immunoprecipitations  for many years and have done IEFs as
> well.  Last I recall, there is not a major chemical difference between NP40
> and TX100 and for doing membrane extractions and IP, they are operationally
> interchangable.  I'm sure I'll get an argument, but in all my years of
> dealing with NP40 vs TX100, I firmly believe you could make the
> substitution without seeing a difference in your experiment.  I'm sure
> there are some applications where the substitution couldn't be made but
> membrane extracionts isn't one of them.

I concur.

Regards,

John A. Newitt, Ph.D.           |   <newitt at removeme.nih.gov>
National Institutes of Health   |   FAX: 301-402-0387
Bethesda, Maryland  USA         |   

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