HELP, How do I control for (prevent) PCR recombination??
dellaire at ODYSSEE.NET
Sat Aug 2 22:32:14 EST 1997
PCR recombination questions:
First the system:
two primers each specific for a different plasmid vector and both
vectors have about 1.3 kb homology to each other
theoretically only when the two vectors recombine can you amplify a 1.3
kb fragment (primers flank deletions in both vectors)
Problem is how to control PCR recombination and thus limit false
1. What parameters should I play with to prevent PCR recombination?
Thanks for all your hints and tips in advance.
e-mail me at dellaire at odyssee.net
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