HELP, How do I control for (prevent) PCR recombination??

Graham Dellaire dellaire at ODYSSEE.NET
Sat Aug 2 22:32:14 EST 1997


PCR recombination questions:

First the system:

two primers each specific for a different plasmid vector and both
vectors have about 1.3 kb homology to each other

theoretically only when the two vectors recombine can you amplify a 1.3
kb fragment (primers flank deletions in both vectors)

Problem is how to control PCR recombination and thus limit false
positives. 

Question:
1. What parameters should I play with to prevent PCR recombination?


Thanks for all your hints and tips in advance.

e-mail me at dellaire at odyssee.net

Cheers,

Graham



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