problem in PCR clonong

Andrei Popov andrei.popov at
Sat Aug 2 17:19:51 EST 1997

czlq at wrote:
> I have been stoped by a simple PCR cloning work.I have chosen pCR.script and pBluescript vectors and cloned my PCR fragments
> into the vector. Everytime I got some white colones ,but none of them have insert.  I was wondering why the  ratio of this false
> conoles  appeared so high ? The PCR product I used are either the original PCR product or PCR product    purified  by ethanol
> precipitation .If anybody can help me solving this problem, I will very appreciate. Thanks a lot in advance.
> lee

I assume that you will solve the problem eventually. Meanwhile, hybridise! 
Having a problem with the efficiency of cloning should not stop one's work :-)

best wishes,

Andrei Popov

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