rbright at emory.edu
Sat Aug 2 12:13:56 EST 1997
I have used the IGEPAL- CA630 successfully. I have noticed no
difference at all from the NP40. I use it for extraction of genomic DNA
from whole blood.
David L. Haviland, Ph.D. wrote:
> At 13:08 8/1/97 GMT, John S. Walker wrote:
> >I'm in a physiology lab that does some 2D-PAGE. Our IEF recipe calls
> >NP-40 but our supplier recently told us that this is no longer
> >available. Is this true or is it our supplier is no longer carrying
> >IF it is true, can anybody suggest a good replacement?
> This is true, and sadly so. However, in lieu of NP40, Sigma
> recommends the
> use of Igepal CA-630, it is chemically (according to Sigma)
> indistinguishiable from NP40. Apparently, NP40 is no longer made.
> not tried Igepal.
> I've been doing immunoprecipitations for many years and have done
> IEFs as
> well. Last I recall, there is not a major chemical difference between
> and TX100 and for doing membrane extractions and IP, they are
> interchangable. I'm sure I'll get an argument, but in all my years of
> dealing with NP40 vs TX100, I firmly believe you could make the
> substitution without seeing a difference in your experiment. I'm sure
> there are some applications where the substitution couldn't be made
> membrane extracionts isn't one of them.
> Hope this helps,
> David L. Haviland, Ph.D.
> Asst. Prof. Immunology
> University of Texas - Houston, H.S.C.
> Institute of Molecular Medicine
> 2121 W. Holcombe Blvd.
> Houston, TX 77030
> Internet:"dhavilan at imm2.imm.uth.tmc.edu"
> Voice: 713.500.2413 FAX: 713.500.2424
> Never take life seriously. Nobody gets out alive anyway.
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