Detecting a one base deletion with SSCP or a PAGE?

Karl Fischer tyr-2 at bones.biochem.ualberta.ca
Sun Aug 3 18:32:43 EST 1997


In article <5s2s60$mss at mserv1.dl.ac.uk>, wgschech at med.uni-tuebingen.de wrote:

Wolfgang,

Why not just perform single lane sequencing on your minipreps? If your
plasmids have an A insert (for example) just perform the ddA reaction on
all your preps
and compare the sequence with your wild-type control. Going to SSCP or
trying to distinguish a 1 nt-longer restriction product on PAGE seems a
bit bullish in this instance.

Just a thought

Karl the hepB guy

-- 
Karl Fischer
tyr-2 at bones.biochem.ualberta.ca





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