Detecting a one base deletion with SSCP or a PAGE?

Karl Fischer tyr-2 at
Sun Aug 3 18:32:43 EST 1997

In article <5s2s60$mss at>, wgschech at wrote:


Why not just perform single lane sequencing on your minipreps? If your
plasmids have an A insert (for example) just perform the ddA reaction on
all your preps
and compare the sequence with your wild-type control. Going to SSCP or
trying to distinguish a 1 nt-longer restriction product on PAGE seems a
bit bullish in this instance.

Just a thought

Karl the hepB guy

Karl Fischer
tyr-2 at

More information about the Methods mailing list