Detecting a one base deletion with SSCP or a PAGE?

Andrey S. Shaw shaw at
Sun Aug 3 23:46:14 EST 1997

Another way to do this is to design a pcr strategy where the last base
pair of one primer is your extra base.  Therefore only your mutated
plasmids should amplify a product.  If the last base of the oligo cannot
pair, the oligo cannot prime.

andrey shaw

More information about the Methods mailing list