Detecting a one base deletion with SSCP or a PAGE?

Andrey S. Shaw shaw at immunology.wustl.edu
Sun Aug 3 23:46:14 EST 1997


Another way to do this is to design a pcr strategy where the last base
pair of one primer is your extra base.  Therefore only your mutated
plasmids should amplify a product.  If the last base of the oligo cannot
pair, the oligo cannot prime.

andrey shaw



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