Isolation of Nuclei and Preparation of Nuclear Extracts??

Graham Dellaire dellaire at ODYSSEE.NET
Sun Aug 3 08:10:26 EST 1997


I am trying to isolate nuclei after transfection of murine fibroblasts
with CaPO4. Does anyone have a reference or a good (easy, lowtech)
protocol for isolating nuclei and fractionating nucleoplasm from nuclear

I want to look at where my vector goes... i.e. is it in the nucleoplasm
or associated with the matrix.

Thanks in advance for all your help,

Graham Dellaire
dellaire at

p.s. if possible please reply via e-mail

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