Koen A.L. De Smet
k.desmet at nospam.ic.ac.uk
Mon Aug 4 07:40:00 EST 1997
Dr.W.H. Colledge wrote:
> We wish to formaldehyde bomb our tissue culture room to eliminate an
> annoying sorce of low level contamination. Does anyone know where we can
> purchase the bomb? Many thanks.
> Dr. W.H. Colledge
> University of Cambridge
> CB2 3EG
> Tel. 01223-333881
> Fax. 01223-333840
We have a containment level three room, which we occasionally have to
bomb with formaldehyde.
For this, we use Laycock Fumigators from Tolbest Ltd, 10 Aston Court,
Kingsland Grange, Warrington, Cheshire WA1 4SG. I presume you can also
use them for tissue cultures.
Just a few extra tips. We make sure we can seal the room up COMPLETELY,
to leave the vapour in it for 24 hours. For this we put masking tape
around both doors (inner andouter door of the lobby) and tape a plastic
bag over the air inlet (windows are always sealed anyway..). The
formaldeyhde doesn't get into our incubators. At the end of the 24
hours, we remove the seals and switch on the cabinets, FROM OUTSIDE THE
ROOM (one cannot go in there without a gasmask).
You can also produce formaldehyde vapour by simply mixing formaldehyde
with potassium permanganate. I am not sure of the ratios, but think it
is around 25 ml and 10 g. This may have to be scaled up to do a whole
room. Don't try this before you have verified these ratios, becuase it
is a rapid exothermic reaction.
I hope this helps, but I am not prepared to take any responsibility if
you have problems with formaldehyde escaping out of the room or any
other hazards associated with this procedure!
To reply by email, remove "nospam." from the above address
Dr Koen A.L. De Smet
Department of Medical Microbiology
Imperial College Medical School at St Mary's
London W2 1PG
Tel: (+44)-(0)171-594 3946
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