Bacterial Expression & ArgU

Gene Holowachuk geneh at TELENET.NET
Mon Aug 4 11:49:26 EST 1997


We are trying to overproduce a mammalian protein in E coli and are having
very poor success. The sequence includes 7 arg codons (3-AGA & 4-AGG) which
are 6% of the total codons. I was wondering if anyone could provide us with
a pACYC-argU plasmid or similar &/or advice. The expression plasmid we are
using is a derivative of pBR322 which would have no compatibility problems
with pACYC plasmid. Please post direct to me as we dont subscribe to the
list. MTIA.

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+  Gene Holowachuk, Ph.D.            .***.           .***.   .***.        +
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