Bacterial Expression & ArgU

[Gene Holowachuk]

We are trying to overproduce a mammalian protein in E coli and are having
very poor success. The sequence includes 7 arg codons (3-AGA & 4-AGG) which
are 6% of the total codons. I was wondering if anyone could provide us with
a pACYC-argU plasmid or similar &/or advice. The expression plasmid we are
using is a derivative of pBR322 which would have no compatibility problems
with pACYC plasmid. Please post direct to me as we dont subscribe to the
list. MTIA.

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+  Gene Holowachuk, Ph.D.            .***.           .***.   .***.        +
+  Research Institute              * | | | *       * | | | * | | | *      +
+  Mary Imogene Bassett Hospital      *| | | *   * | | | *   * | | | *    +
+  One Atwell Road                     * | | | * | | | *       * | | | *  +
+  Cooperstown, NY 13326-1394            '***'   '***'           '***'    +
+                                                                         +
+  Email:"geneh at usa.net" OR "geneh at telenet.net"                           +
+  Voice: 607-547-3937  FAX: 607-547-3061 / 4904                          +
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