A new problem with GST fusion protein

Anne-Laure Provost alprovos at ens-lyon.fr
Fri Aug 1 12:57:52 EST 1997


Hello,
I'm trying to do exprime a GST fusion protein in XL1 blue (E.Coli).
I don't have the protein waited but a smaller, futhermore the 
induction is very weak. I think my protein is cutted because of a 
creation of cleavage site. Do you know site of cleavage in this 
bacteria, do you have solution? Have you test this technique of 
fusion-protein, what do you think about it? What are the best 
conditions of induction?



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