A new problem with GST fusion protein
alprovos at ens-lyon.fr
Fri Aug 1 12:57:52 EST 1997
I'm trying to do exprime a GST fusion protein in XL1 blue (E.Coli).
I don't have the protein waited but a smaller, futhermore the
induction is very weak. I think my protein is cutted because of a
creation of cleavage site. Do you know site of cleavage in this
bacteria, do you have solution? Have you test this technique of
fusion-protein, what do you think about it? What are the best
conditions of induction?
More information about the Methods