Classical transfer buffers are: - Towbin buffer (25mM Tris, 192 mM glycine, 20% methanol, 0.1% SDS) (electrophoresis run buffer plus methanol) - or 48 mM Tris, 39 mM glycine, 0.0375 % SDS, 20% methanol 0.8 mA/cm2 and no problem good luck