semi-dry transfer

Joel Baguet jbaguet at cri.ens-lyon.fr
Tue Aug 5 06:18:24 EST 1997


Classical transfer buffers are:

	- Towbin buffer (25mM Tris, 192 mM glycine, 20% methanol, 0.1% SDS) 
(electrophoresis run buffer plus methanol)
	- or 48 mM Tris, 39 mM glycine, 0.0375 % SDS, 20% methanol

0.8 mA/cm2 and no problem

good luck



More information about the Methods mailing list