Pichia expression system?

James R. Bone jrbone at ODIN.MDACC.TMC.EDU
Wed Aug 6 15:01:27 EST 1997


Is anyone using the Pichia system for expression and purification
heterologous proteins.

Please reply if you have any experience with Pichia.

Do you get more protein than Saccharomyces?
Is the secreted (alpha-factor pre-pro signal peptide) system useful.
Is it as easy to work with as Saccharomyces?
What are the best vectors?
Any disadvantages?



Thanks


JB
-----------------------------------------------------------------------------


James R. Bone
Dept. of Biochemistry and Molecular Biology
M. D. Anderson Cancer Center, Box 117
1515 Holcombe Avenue
Houston, TX 77030

email:jrbone at odin.mdacc.tmc.edu
     :jrbone at onramp.net

voice: 713-792-2549
fax:   713-791-9478

Roth Lab Homepage-- 
        http://rampages.onramp.net/~jrbone/rothlab.htm

Chromatin Structure & Function Page-- 
        http://rampages.onramp.net/~jrbone/chrom.html  


"The truths of organic nature are of a charming and awesome beauty.  The
deeper you penetrate into their details and special features, the more
beautiful they become."

                                               Konrad Lorenz, 1949

----------------------------------------------------------------------------




More information about the Methods mailing list