Pichia expression system?

[James R. Bone]

Is anyone using the Pichia system for expression and purification
heterologous proteins.

Please reply if you have any experience with Pichia.

Do you get more protein than Saccharomyces?
Is the secreted (alpha-factor pre-pro signal peptide) system useful.
Is it as easy to work with as Saccharomyces?
What are the best vectors?
Any disadvantages?



Thanks


JB
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James R. Bone
Dept. of Biochemistry and Molecular Biology
M. D. Anderson Cancer Center, Box 117
1515 Holcombe Avenue
Houston, TX 77030

email:jrbone at odin.mdacc.tmc.edu
     :jrbone at onramp.net

voice: 713-792-2549
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Roth Lab Homepage-- 
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Chromatin Structure & Function Page-- 
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"The truths of organic nature are of a charming and awesome beauty.  The
deeper you penetrate into their details and special features, the more
beautiful they become."

                                               Konrad Lorenz, 1949

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