Isopropanol & EDTA

David MacHugh dmachugh at mail.tcd.ie
Wed Aug 6 10:56:08 EST 1997


 
Hi,

Does anyone know whether very high concentrations of EDTA (~0.25M) will
affect DNA precipitations using isopropanol?  I have noticed that the
aqeuous material seems to separate out as a layer below the isopropanol. 
I haven't tried to recover the DNA yet, but I reckon it won't work.

Any comments..

Thanks in advance.

David MacHugh.

Genetics Department,
TCD.
Ireland.



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