spun column

Elham Ettehadieh ettehadieh at aeiveos.com
Thu Aug 7 01:38:33 EST 1997


Molecular Pathology Lab wrote:
> 
> Hello all-
>         I was wondering in anyone has a protocol for doing a G-50 spun column
> using a microcentrifuge for seperating unicorporated from labled probe.
> I have used the 1mL (tuberculin) syringe method, but I don't have access
> to a clinical centrifuge, but I do have access to an old
> microcentrifuge.  Any help that you can give me will be greatly
> appreciated, and you can either send it to my e-mail or to the news
> group.
> 
> Thanks in advance.
> 
> -Eric W. Olle


We buy ours (G-50 or G-25) from 5'-3' and they are pretty good.  Spin 
them (in a microfuge at top speed) for 90 sec, add your sample, let it 
sit for 2 min and spin for 60 sec.  We have run acrylamide gels on our 
porbes to see how well it works and it seems to work pretty well.  

Good luck.



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