Mme I or TypeIII restirction enzyme

Dr. Duncan Clark duncan at
Thu Aug 7 04:10:28 EST 1997

In article <5saafe$e9h at>, Rifat Hamoudi <rifat at>
>I am looking to use a 20bp cutter or more like MmeI

Mme I is apparently difficult to purify due to low yield. It only has a
recognition sequence of 6bp even though it cuts 20 bases downstream. If
you want long recognition sequences then the intron encoded RE's are the
ones you want ie Sce I.

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
TEl/FAX 01252376288

More information about the Methods mailing list