His-tag purification

LouPass loupass at aol.com
Thu Aug 7 19:14:08 EST 1997


>From: Lynn Hughes <Lynn.Hughes at jcu.edu.au>
>Date: Thu, 31 Jul 1997 15:31:36 +1000
>Message-ID: <33E02338.6DF6 at jcu.edu.au>
>


>Hi all,
>
>I'm soon to start trying to express my protein of interest using a 
>His-tag expression system (PROEX HT from Life Technologies).  I am 
>looking at buying more resin and columns for the purification.  Has 
>anyone had experience with the various resins available and which are 
>best?  I have come across ProBond Metal Binding Resin, Talon Metal 
>Affinity Resin, His-Bind Resin and the Ni-NTA resin from Quiagen.  Any 
>comments would be appreciated.
>
>Lynn Hughes
>Dept. Biochemistry & Molecular Biology
>James Cook University
>Australia

Have used the Ni-NTA resin from Qiagen with good success.  Their tips are
very useful if you run into problems.   


Lou Passador
Dept. of Microbiology and Immunology
Univ. of Rochester
Rochester NY.



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