Free trial of GreenstoneTM 750 for PCR DNA cleanup
bowers at shore.net
Thu Aug 7 16:55:04 EST 1997
We have developed a high performance reusable microfuge UF membrane concentrator Kit which is
designed for more cost-effective purification and concentration of proteins (>10 kD) and of
nucleic acids. Studies with proteins and PCR-amplified 418bp DNA have demonstrated quantititive
recovery, and a deadstop which absolutely prevents filtration to dryness. 100x concentration of
up to 750uL can be done in 15-45 min, depending on the membrane pore size selected.
We need to characterize the ds DNA bp retention limits of our more open membranes which have
been shown to retain <95% of globular proteins of 70kD and 150kD, respectively. This cannot be
done meaningfully with commercial DNA ladder mixtures, due to the enhanced retention of
slightly smaller DNA by the polarized retained larger DNA fractions.
To that end we are offering 2 free devices preassembled with the 70kD membrane to anyone who
is doing PCR directed to amplifying DNA in the size range of 60-120bp, and 2 devices assembled
with the 150kD membrane to anyone amplifying DNA of 100-500bp or >10,000bp. In return for a gel
photo showing separation of labled primers from amplified product (eq ladder mixture, unpurified
PCR reaction, first filtrate, and second TE-buffer-washed retentate) we will offer a credit of
$60 (half price) toward the purchase of a complete System Kit (4 device sets, tubes and caps, 80
membranes of up to 4 different retention limit ratings, assembly tool, and a cleaning/storage
If you are interested, please Email directly to bowers at shore.net with your mailing address
and we will send devices with an Operating Guide, suggested diafiltration protocol and a
discount credit certificate.
More information about the Methods