Cultured cells at constant pH?

Ian A. York iayork at
Thu Aug 7 10:27:40 EST 1997

In article <33E9F047.5615 at>,
Dom Spinella <dspinella at,> wrote:
>The problem with most culture media using the common CO2/bicarbonate
>buffering system is that bicarb is a very weak buffer at physiological
>pH.  A better choice is one of the Good buffers such as HEPES (at 10 -
>20 mM) which is a much stronger buffer at this pH range.  See R. Ian

I've been using HEPES-buffered media, and the pH still drops dramatically
as the cells grow, because they all have some bicarbonate buffering as
well.  Thanks for the recommendation of the book, though; I'll check it

      Ian York   (iayork at  <>
      "-but as he was a York, I am rather inclined to suppose him a
       very respectable Man." -Jane Austen, The History of England

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