beta-mercaptoethanol and transfromation
David R. Johnson
johnson at biomed.med.yale.edu
Thu Aug 7 06:25:31 EST 1997
> > > Can I know the role of beta-mercatoethanol in trasformation of E.coli?
(Yes Geetha, if I knew, I would certainly tell you the role...)
According to GIBCO/BRL literature, transformation of DH5 cells is not increased by addition of
beta-mercatoethanol, so any hypotheses about how it works could be formulated or tested with this in
mind. I second the recommendation of the Hanahan (the DH of DH5) paper on transformation techiques.
> Hanahan, D. Studies on Transformation of Escherichia coli with Plasmids,
> J. Mol. Biol. 166 : 557-580 (1983)
> David F. Spencer
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