Questions on Daltons and ELISA
Pascal.Mertens at fundp.ac.be
Fri Aug 8 08:12:09 EST 1997
In article (Dans l'article) <5sdv82$h55$1 at nntp.ucs.ubc.ca>,
dcwong at unixg.ubc.ca (Donald Chun Kit Wong) wrote (écrivait) :
> I have two question. First, can anyone tell me the relationship between
> daltons and grams. Can one calculate one from the other?
1 mole (6 x 10E23 molecules) of a N daltons molecule weights N grams.
> Second, when coating a plastic microtiter plate with antibodies, will
> only the Fc portion of the antibody bind to the plate, or only the F(ab)
> portion, or all parts of the antibody equally?
Coating is a non-specific adsortion to the plastic. So, parts of the Ab
binded to the plates are probably equally distributed on the surface of
> companies, texts and review articles always show only the antibodies
> bound by the Fc portion. But never mention if other portions of the
> antibody can bind also.
It's probably shown that way because Ab are not directly coated to the
plate, but are binded to coated Protein G or Protein A.
Hope this helps
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