MY PCR product
MBQ96AA at shef.ac.uk
Fri Aug 8 11:42:11 EST 1997
I wonder if any one could find a suitable solution for a PCR product of
2Kb gene I designed a SmaI site before 5'end of this PCR product and
EcoRI before 3'end of it. Then I ligated this blunt end PCR
product(because of using Vent Polymerase) in PBR322 plasmid at EcoRV site
but when I tried to recover my PCR product by digestion with SmaI then
EcoRI it wasn't cutted with SmaI!
Note : I have tested SmaI enzyme and it was alright.
Many thanks for your help
PhD student at University of Sheffield
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