Lysozyme activity?

Dr. Duncan Clark duncan at genesys.demon.co.uk
Fri Aug 8 04:37:15 EST 1997


In article <33ea9a7f.9331876 at usenet.seri.re.kr>, Yeon Chul Kim
<e_yckim at cais.kaist.ac.kr> writes
>I'm using E. coli containg pLysS gene.
>I want to check how many lysozyme produce by pLysS gene.
>So, I'd like to know how to check the lysozyme activity simply.
>Please letme know.

If you just want to know if it is active then freeze-thaw of an E.coli
strain containing pLysS producing T7 lysozyme will cause lysis. Without
active T7 lysozyme the same strain should not lyse.

Duncan 

-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
TEl/FAX 01252376288
http://www.dnamp.com
http://www.genesys.demon.co.uk



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