Lysozyme activity?

Dr. Duncan Clark duncan at
Fri Aug 8 04:37:15 EST 1997

In article <33ea9a7f.9331876 at>, Yeon Chul Kim
<e_yckim at> writes
>I'm using E. coli containg pLysS gene.
>I want to check how many lysozyme produce by pLysS gene.
>So, I'd like to know how to check the lysozyme activity simply.
>Please letme know.

If you just want to know if it is active then freeze-thaw of an E.coli
strain containing pLysS producing T7 lysozyme will cause lysis. Without
active T7 lysozyme the same strain should not lyse.


The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
TEl/FAX 01252376288

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