5-FOA/2+3 hybrid

Jan Liphardt jtcl3 at cam.ac.uk
Sat Aug 9 17:26:15 EST 1997


Hi,

I'm not sure if this is current, but the Genetics Society of America
(Bethesda, Md.) used to sell discounted 5-FOA to its menbers in bulk.  The
Sigma stuff works just fine, though. For some applications which would
otherwise be absurdly expensive (like removing a URA plasmid from 200 clones)
try growing twice in 1.5 ml liquid cultures, and use 500 microL of the second
culture to test for B-gal.  Works every time. 

Hope this helps,

Jan

Dept of Pathology
Cambridge University
UK



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