How to remove DNA from mRNA...???

ISOAI Atsushi aisoai at agc.co.jp
Sat Aug 9 12:20:21 EST 1997


lumdicks at netvigator.com wrote:

>> I'm about to use RT-PCR to demonstrate induction of an
>> operon. This puts me in a similar position of having to 
>> avoid amplification of DNA from the total RNA prep.
>>
>> The route I'll be using is to treat the RNA prep with DNAse
>>prior to amplification. I've ordered some RNase-free DNAse 
>>to do it. I haven't tried this yet myself, but others in our
>>lab have used it successfully
>> on prokaryotic RNA preps.
>
>But how can I safely inactivate the DNase after the
>treatment?? Trace amount of it will certain ruin the
>whole experiment.

Hi, Dick;
You can inactivate DNasae by heat treatment (80 C-15min)
or by phenol treatment. The both are very basic mathods:-).
bye

-- 
====================================================
 Atsushi Isoai, Ph.D.    |   <aisoai at agc.co.jp>
 Research Center, Asahi Glass Co.LTD., JAPAN
 URL: http://www.yk.rim.or.jp/~aisoai/index.html
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