How to remove DNA from mRNA...???

Dickens Lum lumdicks at netvigator.com
Sat Aug 9 10:42:10 EST 1997


> I'm about to use RT-PCR to demonstrate induction of an operon. This
> puts
> me in a similar position of having to avoid amplification of DNA from
> the total RNA prep.
>
> The route I'll be using is to treat the RNA prep with DNAse prior to
> amplification. I've ordered some RNase-free DNAse to do it. I haven't
> tried this yet myself, but others in our lab have used it successfully
>
> on prokaryotic RNA preps.

        But how can I safely inactivate the DNase after the
treatment..?? Trace amount of it will certain ruin the whole experiment.

Regards,
Dickens Lum




More information about the Methods mailing list