How to remove DNA from mRNA...???
lumdicks at netvigator.com
Sat Aug 9 10:42:10 EST 1997
> I'm about to use RT-PCR to demonstrate induction of an operon. This
> me in a similar position of having to avoid amplification of DNA from
> the total RNA prep.
> The route I'll be using is to treat the RNA prep with DNAse prior to
> amplification. I've ordered some RNase-free DNAse to do it. I haven't
> tried this yet myself, but others in our lab have used it successfully
> on prokaryotic RNA preps.
But how can I safely inactivate the DNase after the
treatment..?? Trace amount of it will certain ruin the whole experiment.
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