Dissolving Amino Acids in Scintillation Fluid
Phil Calvert
calvert at eos.ncsu.edu
Tue Aug 12 01:49:37 EST 1997
I hydrolyzed some protein with HCl and passed the hydrolyzate through a
small ion-exchange column. The amino acids were then eluted with 4M
ammonium hydroxide. When I tried mixing this with scintillation fluid
(Ultima Gold) it became very cloudy. I figured it must be the ammonia, so
I blew it down to dryness with nitrogen. Well, I still couldn't get it to
dissolve properly. This makes sense because amino acids don't dissolve
very well in organic solvents, generally speaking. Is there a trick to
this? I was thinking that maybe using a co-solvent like DMSO, DMF,
pyridine, etc. might work, but this is just a hunch. Any suggestions?
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