INVITROGEN pCR2.1-TOPO PROBLEMS

WILLIAM M GROSSE GROSSE.M at CALC.VET.UGA.EDU
Mon Aug 11 04:12:27 EST 1997


I want to know if anyone has had any problems with INVITROGEN's
pCR2.1-TOPO kit?  I finally got a 400 bp PCR product cloned into the
vector but one of the restriction sites that were synthesized on the 5'
ends of both primers will not cut.  Also, some of the restriction sites in
the vector's poly-linker do not seem to cut.  Finally, the linerized
vector seems to be at least twice the size that the vector is supposed to
be (3.9 kb).  THANKS

MIKE GROSSE
DEPT. OF PHYS/PHARM
UNIVERSTIY OF GEORGIA
GROSSEM at CALC.VET.UGA.EDU
(706) 542-0648



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