pCR-Script Kit

Rick Davis richard..davis at worldnet.att.net
Tue Aug 12 08:05:33 EST 1997

Could someone please explain to me the reasoning behind the Stratagene
pCR-Script kit for cloning blunt-ended PCR products? I don't
understand how one can achieve efficient cloning of PCR products
that don't have phosphates on their 5' ends. Does the transforming
cell repair the vector/insert gap  resulting from ligation of a
phosphorylated vector to a non-phosphorylated PCR insert?


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