DNA Recombination\duplication assay
cwh at staff.monash.edu.au
cwh at staff.monash.edu.au
Tue Aug 12 01:50:59 EST 1997
Hi people
I am trying to design a recombination assay of some sort to detect duplication
of a large region of DNA in a bacteria. I have a few putative recombinases
cloned and am hoping to overexpress them in a background that will select for
duplication (presumably using a marker gene of some kind). It sounded easy
but is harder in practice than in theory. Any help would be greatly
appreciated
chris
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