pCR-Script Kit

Dr. Duncan Clark duncan at genesys.demon.co.uk
Tue Aug 12 11:37:53 EST 1997


In article <33f05e39.720960 at netnews.worldnet.att.net>, Rick Davis
<richard.?.davis at worldnet.att.net> writes
>Does the transforming
>cell repair the vector/insert gap  resulting from ligation of a
>phosphorylated vector to a non-phosphorylated PCR insert?

Yes - same logic behind construction of gene banks using a phosphatased
vector and phosphorylated inserts.

Duncan
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
TEl/FAX 01252376288
http://www.dnamp.com
http://www.genesys.demon.co.uk



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