RNase inhibitor - thermostability?

Björn Rosén bjornrosen at swipnet.se
Wed Aug 13 14:08:31 EST 1997


Hi,

I have a question regarding thermostability of RNase inhibitors. 

The RNA extraction protocol ends with an incubation at 60 degrees
centrigrade. The extracts will then be cooled to RT (or frozen) before I
attempt RT-PCR. My worry is that the RNase inhibitor denatures during
the incubation and that RNases revive when the temperature is lowered.

The RNase inhibitor used is Perkin Elmer's P/N N808-0119.

Does anyone know?

Thanks, Bjorn



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