HELP: HOT cDNA PROBE

Qin Zong qzong at u.washington.edu
Wed Aug 13 15:48:15 EST 1997


I'm going to make some  cDNA probes from mRNA by using BRL's Superscript
kit. I'd like to make them as hot as possible. So I'm going to reduce
the cold dCTP conc. in the dNTPs mixture (I'm going to us hot dCTP,
3,000 mCi/mmol; 10mCi/ml). Can anybody tell me how much cold dCTP you
added into the reaction mixture when you make the high SA cDNA probe? 

Thank you very much.

Qin



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