PCR purification KITS: Best one??????
mikel at bioc.rice.edu
Wed Aug 13 14:34:50 EST 1997
Can anyone help me... I am at that point. I perform PCR reactions and I
get one specific product. I have used the Qiagen clean up kit, the
Geneclean Kit, and I have done a good ol ammonium acetate precipitation.
What I see is conversion to what presumably is a single stranded form. I
have repeated the purification and I get the same result consistently. I
rang the Qiagen reps and they told me that for DNA that has some unique
characteristic (such as high A+T content), there is some conversion to a
single stranded form. I am having an immense amount of trouble with it.
I am also wasting a lot of time with trouble shooting this problem which
exists for 4 independent PCR reactions.
Has anyone any suggestions that may be of some help. I do not know what
Department of Biochemistry and Cell Biology
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