PCR purification KITS: Best one??????

[Mike Lyristis]

Hello there,

Can anyone help me... I am at that point. I perform PCR reactions and I
get one specific product. I have used the Qiagen clean up kit, the
Geneclean Kit, and I have done a good ol ammonium acetate precipitation.
What I see is conversion to what presumably is a single stranded form. I
have repeated the purification and I get the same result consistently. I
rang the Qiagen reps and they told me that for DNA that has some unique
characteristic (such as high A+T content), there is some conversion to a
single stranded form. I am having an immense amount of trouble with it.
I am also wasting a lot of time with trouble shooting this problem which
exists for 4 independent PCR reactions.

Has anyone any suggestions that may be of some help. I do not know what
to do...


Many thanks
Mike L
Department of Biochemistry and Cell Biology
Rice University
Houston, TX,
USA