hot-start PCR

microbio at microbio at
Fri Aug 15 09:51:32 EST 1997

Hot-started PCR was performed with paraffin wax bead.
It's amplification efficiency was lower than manual hot-start PCR
(after denaturation step, open tube cap and add Taq.pol)
Any thoughts about this results?
I think this result was due to Taq. pol degeneration during
degeneration step or uncomplete melting of wax-layer..
but I can't sure..

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