Liver DNA

John C. Robinson jcrobin at welchlink.welch.jhu.edu
Wed Aug 13 17:23:23 EST 1997


> Helene Bach wrote:
> >
> > Does anyone how to isolate DNA from liver? (My preps have alot off
> > pigment).
> 
For what it's worth, we have had many extractions which yielded
pigmented DNA solutions, and the resulting DNA has worked fine for our
standard Southerns and PCRs.  Liver, like seminal vesicle, tends to have
a great deal of crud in it - I've had to occasionally do 4 or even 5
phenol/ClI extractions to get the solution clean, but otherwise it works
as well as any other DNA for what we do.
Reg'ds,
	JR



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