Histidine tag fusion protein

[Koen A.L. De Smet]

slavica masina wrote:
> 
> I am currently performing bacterial cell expressions with a histidine tag
> fusion protein (~50kDa) and am having some trouble purifying the
> expressed products using a talon metal affintiy resin under reducing (8M
> Urea) and native conditions. Does anyone have any sugestions for
> successful purification of His fusion proteins? Or any ideas on ow to
> improve the talon affintiy metod? Thanks Slavica

Can you specify whatthe "problem" is. It may help the newsgroup to give 
relevant answers!
is the protein expressed?
does it bind to the resin?
etc...

Koen

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        Dr Koen A.L. De Smet
        Research Fellow
        Department of Medical Microbiology
        Imperial College Medical School at St Mary's
        Norfolk Place
        London W2 1PG
        Great Britain
                                     
        Tel: (+44)-(0)171-594 3946  
        Fax: (+44)-(0)171-262-6299   
        http://www.sm.ic.ac.uk/medmicro/home.htm
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