Lysozyme activity?

June Eva Paciga jpaciga at com1.med.usf.edu
Thu Aug 14 12:32:31 EST 1997


Yeon Chul Kim wrote:
> 
> Hi!!
> I'm using E. coli containg pLysS gene.
> I want to check how many lysozyme produce by pLysS gene.
> So, I'd like to know how to check the lysozyme activity simply.
> Please letme know.
> It would be greatly appreciate.
> Thank you for in advance.
> 
> Yeon Chul Kim

You can use the spectrophotometric lysozyme activity assay, an assay
which measures lysozyme activity by its ability to degrade Micrococcus
lysodeikitcus. Point to the following Sigma Technical Insert:
  
http://www.sigald.sial.com/sigma/enzymes/lysozyme.htm

In addition I mailed the information directly to you.

I hope this helps.

June
-- 
June Eva Paciga
Univ South Florida-Pathology
Tampa FL
jpaciga at com1.med.usf.edu



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