Obtaining DNA from formalin fixed tissue
W.T Howson
whowson at julian.uwo.ca
Thu Aug 14 12:30:44 EST 1997
Does anyone out in this group have any experience in obtaining DNA from
formalin fixed/parrafin embedded tissue. We have tried two methods,
proteinase K treatment and a modified Qiagen tissue kit after removing
the parafin using standard technique and appear to get no DNA from these
tissues. Are we doing something wrong or is there a more reliable and sure
method available.
thanks
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