Help: SP6/T7 specificity

Bernard Murray, PhD bernard at elsie.nci.nih.gov
Fri Aug 15 22:26:53 EST 1997


In article <33F4DD91.64CF at ubaclu.unibas.ch>, schuchert at ubaclu.unibas.ch wrote:

> How specific are SP6 and T7 RNA polymerase concering the other
> promoter sequences?
> 
> I use RNA trancripts as in situ probes, generated from an cDNA
> with flanking T7 and SP6 promoters. The antisense probe gives
> me a very strong signal in the expected cell population. However,
> also the sense probe gives a signal in the same cells, although 
> much much weaker.
> I inerpreted this that by generating my sense probe with SP6,
> also some transcripts are formed from the T7 site and producing.



More information about the Methods mailing list